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Clone Selection | Stages | Sanitary Selection | Agronomic Selection  
 
  Sanitary selection  
   
Sanitary selection is one of the two elements of clonal selection and is carried out in parallel to the agronomic selection.
N.B.: Only the sanitary selection phase is carried out for the rootstock varieties.

Detection of viral diseases
  INDICATIVE
VARIETIES		 SEROLOGY
 MAIN VIRUS DISEASES
    Fan Leaf : 2 types
  • Grapevine Fanleaf Virus - GFLV
  • Arabis Mosaïc Virus - ArMV
 Rupestris du Lot
GFLV
ArMV
CNa
    Leaf Roll : 3 types
  • Grapevine Leafroll associated Virus
Cabernet franc N
Cabernet-Sauvignon N
Pinot noir N
GLRaV-1
GLRaV-2
GLRaV-3
    Fleck
  • Grapevine Fleck Virus - GFkV
 Rupestris du Lot GFkV

 SECONDARY VIRAL DISEASES
    Rugose wood complex (or striations)
  • Rupestris Stem Pitting - RSP
  • Kober Stem Grooving - KSG
  • Grapevine Corky Bark - GCB
 Rupestris du Lot
5BB
LN33		 GVA
    Vein Necrosis
  • Grapevine Vein Necrosis - GVN
 110 R  
    Vein Mosaic
  • Grapevine Vein Mosaïc - GVM
 Riparia Gloire  

Symptoms of Fan Leaf and Rupestris du Lot

Detection methods
  1 - BIOLOGICAL TESTING : INDEXING



This involves grafting a scion of a clone to be tested onto a cutting of an indicator variety which will present the symptoms of the virus in question if it is present.



Indexing can be done with wood grafting (3 years) or greengrafting (6 to 12 months). This is the method of reference for clonal certification. 		  Herbaceous Grafting Herbaceous Indexing
 

2 - SEROLOGICAL TEST - ELISA
(Enzyme Linked Immuno Sorbent Assay)

Immuno enzymatic technique based on an antibody (serum), antigen (virus) reaction.

The ELISA test is used as a control test for monitoring the sanitary status during the propagation process. 		  ELISA test plate
 

3 - MOLECULAR TEST - PCR

Virus detection using specific molecular markers (primers) by genomic amplification after reverse transcription (RT –PCR) or immuno-capture reverse transcription (IC-RT-PCR).


  Treatment Method


It is mainly used for varieties from which healthy clones cannot be obtained by sanitary selection or for clones which carry viruses but which present a special agronomic interest.

Removal of the apex from a virus carrying plant and grafting in vitro onto a hypocotyl of a seedling plant. 		  apex microgreffé sur hypocotyle de semis
 
   
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